12753-1-AP

antibody from Invitrogen Antibodies

Targeting: PDE12 2'-PDE, 3635, DKFZp667B1218

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12753-1-AP

Provider

Invitrogen Antibodies

Product name

PDE12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KLYYREEAV AEDVLNVDAW QDGAVLQIGD VKYKVERNPP AFTELQLPRY IMAGFPVCPK LSLEFGDPAS SLFRWYKEAK PGAAEPEVGV PSSLSPSSPS SSWTETDVEE RVYTPSNADI GLRLKLHCTP GDGQRFGHSR ELESVCVVEA GPGTCTFDHR HLYTKKVTED ALIRTVSYNI LADTYAQTEF SRTVLYPYCA PYALELDYRQ NLIQKELTGY NADVICLQEV DRAVFSDSLV PALEAFGLEG VFRIKQHEGL ATFYRKSKFS LLSQHDISFY EALESDPLHK ELLEKLVLYP SAQEKVLQRS SVLQVSVLQS TKDSSKRICV ANTHLYWHPK GRFYLVSQVT (124-472 aa encoded by BC034978)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.14 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug.