PA5-112063

antibody from Invitrogen Antibodies

Targeting: LILRA1 CD85i, LIR-6, LIR6

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112063

Provider

Invitrogen Antibodies

Product name

LILRA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of LILRA1 using LILRA1 Polyclonal Antibody (Product # PA5-112063) at a dilution of 1:500. Lane A: MCF7 Membrane Lysate , (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of LILRA1 was performed on (Lane A) 0.5 mg MCF-7 whole cell lysate using 2 µL of LILRA1 Polyclonal Antibody (Product # PA5-112063) at a dilution of 1:100, and 15 µL of 50 % Protein G agarose. A Clean Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1000. Developed using the DAB staining technique and performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 70 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of LILRA1 was performed on (Lane A) 0.5 mg MCF-7 whole cell lysate using 2 µL of LILRA1 Polyclonal Antibody (Product # PA5-112063) at a dilution of 1:100, and 15 µL of 50 % Protein G agarose. A Clean Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1000. Developed using the DAB staining technique and performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 70 kDa.