GTX102281

antibody from GeneTex

Targeting: TUBGCP2 GCP2, SPBC97, Spc97p

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX102281

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102281, RRID:AB_1952444

Product name

GCP2 antibody [N1N2], N-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma.

Dráberová E, D'Agostino L, Caracciolo V, Sládková V, Sulimenko T, Sulimenko V, Sobol M, Maounis NF, Tzelepis E, Mahera E, Křen L, Legido A, Giordano A, Mörk S, Hozák P, Dráber P, Katsetos CD
Journal of neuropathology and experimental neurology 2015 Jul;74(7):723-42

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX102281 diluted at 1:2000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: Hela 7.5% SDS PAGE GTX102281 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term (GTX102281) diluted at 1:500.Blue: Hoechst 33342 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCP2(GTX102281) antibody at 1:500 dilution.