12393-1-AP

antibody from Invitrogen Antibodies

Targeting: COPG1 COPG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12393-1-AP

Provider

Invitrogen Antibodies

Product name

COPG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RDRATFYLN VLEQKQKALN AGYILNGLTV SIPGLERALQ QYTLEPSEKP FDLKSVPLAT APMAEQRTES TPITAVKQPE KVAATRQEIF QEQLAAVPEF RGLGPLFKSS PEPVALTESE TEYVIRCTKH TFTNHMVFQF DCTNTLNDQT LENVTVQMEP TEAYEVLCYV PARSLPYNQP GTCYTLVALP KEDPTAVACT FSCMMKFTVK DCDPTTGETD DEGYEDEYVL EDLEVTVADH IQKVMKLNFE AAWDEVGDEF EKEETFTLST IKTLEEAVGN IVKFLGMHPC ERSDKVPDNK NTHTLLLAGV FRGGHDILVR SRLLLLDTVT MQVTARSLEE LPVDIILASV G (350-699 aa encoded by BC020498)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human colon tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 12393-1-AP (COPG antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-COPG (IP:12393-1-AP, 4ug; Detection:12393-1-AP 1:2000) with K-562 cells lysate 3600ug.