MA5-31882

antibody from Invitrogen Antibodies

Targeting: RAB6B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31882

Provider

Invitrogen Antibodies

Product name

RAB6B Monoclonal Antibody (6D12E4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

6D12E4

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAB6B in C6 (1), HT-29 (2), and PC-12 (3) cell lysate. Samples were incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAB6B in human Rab6b recombinant protein. Sample was incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAB6B in HEK293 (1) and Rab6b cell lysate. Samples were incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:500-1:2000.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of RAB6B in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of RAB6B in Hela cells (green). Sample was incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of RAB6B in HepG2 cells (green). Sample was incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB6B in paraffin-embedded lung cancer tissue. Sample was incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB6B in paraffin-embedded ovarian cancer tissue. Sample was incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of RAB6B in HeLa cells (green). Sample was incubated with RAB6B monoclonal antibody (Product # MA5-31882) using a dilution of 1:200-1:400 followed by negative control (red).