- PA5-38036 - Invitrogen Antibodies NOX3 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of NOX3 in 293 cell lysate using a NOX3 polyclonal antibody (Product # PA5-38036) at a dilution of 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NOX3 in 293 cell lysate with NOX3 Polyclonal Antibody (Product # PA5-38036) at 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of NOX3 in human kidney tissue using a NOX3 polyclonal antibody (Product # PA5-38036) at a dilution of 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nox3 (Violet) in mouse bone marrow cells (Up). The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product # PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Cy5 (Product # A10523) at a dilution of 1:1000 for 1 hours at RT (panel b: Violet) and wash 5 times with 1xPBS before overlaid with cover slip. Nuclei (panel a: blue) were stained with DAPI (Product # D1306) during the blocking step. Panels a and b are a merged image and magnified squire area, respectively. Images were taken on a Nikon A1 confocal microscopy at 60x Objective lens with oil used. Data courtesy of Dr. Zhi Wen from the University of Wisconsin, Madison.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nox3 (Red) in human G-CSF mobilized CD34+ cells. The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product # PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Dylight 594 (Product # 35560) at a dilution of 1:1000 for 1 hours at RT (panel b: red) and wash 5 times with 1xPBS before overlaid with cover slip. Nuclei (panel a: blue) were stained with DAPI (Product # D1306) during the blocking step. Panel c was the bright field. Panels a, b and c are a merged image and magnified squire area, respectively. Images were taken on a Nikon A1 confocal microscopy at 60x Objective lens with oil used. Data courtesy of Dr. Zhi Wen from the University of Wisconsin, Madison.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of NOX3 in human kidney tissue with NOX3 Polyclonal Antibody (Product # PA5-38036) at 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of NOX3 in human kidney tissue with NOX3 Polyclonal Antibody (Product # PA5-38036) at 20 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of NOX3 in human kidney tissue with NOX3 Polyclonal Antibody (Product # PA5-38036) at 5 µg/mL.

PA5-38036