19143-1-AP
antibody from Invitrogen Antibodies
Targeting: PLCH1
DKFZp434C1372, KIAA1069, MGC117152, PLCeta1, PLCL3
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 19143-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLCH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SRGWLPKSP TKGEDWETLK SCSPASSPDL TLEDVIADPT LCFNSGESSL VEIDGESENL SLTTCEYRRE GTSQLASPLK LKYNQGVVEH FQRGLRNGYC KETLRPSVPE IFNNIQDVKT QSISYLAYQG AGFVHNHFSD SDAKMFQTCV PQQSSAQDMH VPVPKQLAHL PLPALKLPSP CKSKSLGDLT SEDIACNFES KYQCISKSFV TTGIRDKKGV TVKTKSLEPI DALTEQLRKL VSFDQEDNCQ VLYSKQDANQ LPRALVRKLS SRSQSRVRNI ASRAKEKQEA NKQKVPNPSN GAGVVLRNKP SAPTPAVNRH STGSYIAGYL KNTKGGGLEG RGIPEGACTA LHYGHVDQFC SDNSVLQTEP SSDDKPEIYF LLRL (1273-1655 aa encoded by BC113950)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 19143-1-AP (PLCH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19143-1-AP (PLCH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PLCH1 antibody 19143-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PLCH1 antibody 19143-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PLCH1 antibody 19143-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 19143-1-AP (PLCH1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 19143-1-AP (PLCH1 antibody) at dilution of 1:50 (under 40x lens).