14274-1-AP

antibody from Invitrogen Antibodies

Targeting: GFM1 EFGM, EGF1, GFM, mtEF-G1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14274-1-AP

Provider

Invitrogen Antibodies

Product name

GFM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RMHADMMED VEEVYAGDIC ALFGIDCASG DTFTDKANSG LSMESIHVPD PVISIAMKPS NKNDLEKFSK GIGRFTREDP TFKVYFDTEN KETVISGMGE LHLEIYAQRL EREYGCPCIT GKPKVAFRET ITAPVPFDFT HKKQSGGAGQ YGKVIGVLEP LDPEDYTKLE FSDETFGSNI PKQFVPAVEK GFLDACEKGP LSGHKLSGLR FVLQDGAHHM VDSNEISFIR AGEGALKQAL ANATLCILEP IMAVEVVAPN EFQGQVIAGI NRRHGVITGQ DGVEDYFTLY ADVPLNDMFG YSTELRSCTE GKGEYTMEYS RYQPCLPSTQ EDVINKYLEA TGQLPVKKGK AKN (400-751 aa encoded by BC049210)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 14274-1-AP (GFM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 14274-1-AP (GFM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14274-1-AP ( GFM1 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug.