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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-27302 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7.
- Concentration
- 1 mg/mL
Submitted references E2F1 Drives Breast Cancer Metastasis by Regulating the Target Gene FGF13 and Altering Cell Migration.
Hollern DP, Swiatnicki MR, Rennhack JP, Misek SA, Matson BC, McAuliff A, Gallo KA, Caron KM, Andrechek ER
Scientific reports 2019 Jul 24;9(1):10718
Scientific reports 2019 Jul 24;9(1):10718
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGF13 using 30 µg of MCF-7 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a FGF13 polyclonal antibody (Product # PA5-27302) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGF13 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a FGF13 Polyclonal Antibody (Product # PA5-27302) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FGF13 Polyclonal Antibody detects FGF13 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. FGF13 Polyclonal Antibody (Product # PA5-27302) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using FGF13 (Product # PA5-27302) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using FGF13 (Product # PA5-27302) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using FGF13 (Product # PA5-27302) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Sequence trace and alignment for CRISPR-mediated Fgf13 knockout. ( A ) Sequence trace for Fgf13 WT cells and Fgf 13 knockout clone 2H5. ( B ) Western blot analysis for Fgf13 WT cells, Fgf13 knockout cells, and Fgf13 add back cells.
