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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [2]
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- Product number
- MA5-24216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGSF3 Monoclonal Antibody (503621)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human (rh) IGSF4, 4B, 4C, 4D or 8 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 503621
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of mouse Anti-human IGSF3 Monoclonal Antibody (Product # MA5-24216) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGSF3 in human lung tissue. Samples were incubated in IGSF3 monoclonal antibody (Product # MA5-24216) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 human lung carcinoma cell line was stained with mouse Anti-human IGSF3 Monoclonal Antibody (Product # MA5-24216, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IGSF3 in A549 human lung carcinoma cell line. Samples were incubated in IGSF3 monoclonal antibody (Product # MA5-24216) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody.
