Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-48059 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGSF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IGSF4B, rhIGSF4C, and rhIGSF8 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human IGSF3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48059) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGSF3 in human lung tissue. Samples were incubated in IGSF3 polyclonal antibody (Product # PA5-48059) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGSF3 in human lung tissue. Samples were incubated in IGSF3 polyclonal antibody (Product # PA5-48059) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 human lung carcinoma cell line was stained with Sheep Anti-human IGSF3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48059, filled histogram) or control antibody (open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IGSF3 in A549 human lung carcinoma cell line. Samples were incubated in IGSF3 polyclonal antibody (Product # PA5-48059) or control antibody followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.