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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- MA5-27854 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNX27 Monoclonal Antibody (1C6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive Control: 293T, A431, HeLa, HepG2
- Antibody clone number
- 1C6
- Concentration
- 1.37 mg/mL
Submitted references Quantitative fragmentomics allow affinity mapping of interactomes.
Gogl G, Zambo B, Kostmann C, Cousido-Siah A, Morlet B, Durbesson F, Negroni L, Eberling P, Jané P, Nominé Y, Zeke A, Østergaard S, Monsellier É, Vincentelli R, Travé G
Nature communications 2022 Sep 17;13(1):5472
Nature communications 2022 Sep 17;13(1):5472
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNX27 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with SNX27 monoclonal antibody (Product # MA5-27854) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNX27 antibody detects SNX27 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SNX27 antibody SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854) diluted at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNX27 antibody detects SNX27 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SNX27 antibody SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854) diluted at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of cos7 cell transfected with SNX27 using SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854).+: Transfected.--: Endogenous.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SNX27 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854) diluted at 1:200. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of cos7 cells using SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854). Red: Primary antibody. Green: Polyclonal SNX27 antibody. Yellow: Merged color of red and green.
