HPA005949

antibody from Atlas Antibodies

Targeting: AKAP4 AKAP82, CT99, Fsc1, hAKAP82, HI, p82

Antibody data

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Validation data

Product number: HPA005949 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA005949, RRID:AB_1078120
Product name: Anti-AKAP4
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: SALKLIQYHLTQQTKGKDTCEEDCPGSTMGYMAQS TQYEKCGGGQSAKALSVKQLESHRAPGPSTCQKEN QHLDSQKMDMSNIVLMLIQKLLNENPFKCEDPCEG ENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCT SGMKQ
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

AKAP4 protein structure - HPA005949 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and endometrium tissues using Anti-AKAP4 antibody. Corresponding AKAP4 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human colon, kidney, liver and testis using Anti-AKAP4 antibody HPA005949 (A) shows similar protein distribution across tissues to independent antibody HPA020046 (B).
Antibody #2 product nr: HPA020046
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows moderate positivity in spermatids.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human endometrium shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-AKAP4 antibody HPA005949.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-AKAP4 antibody HPA005949.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-AKAP4 antibody HPA005949.
Sample type: HUMAN