AMAb90856

antibody from Atlas Antibodies

Targeting: DIAPH2 DIA, DIA2, POF, POF2

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb90856

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90856, RRID:AB_2665693

Product name

Anti-DIAPH2

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

GGSEEPGGGRSNKRSAGNRAANEEETKNKPKLNIQ
IKTLADDVRDRITSFRKSTVKKEKPLIQHPIDSQV
AMSEFPAAQPLYDERSLNLSEKEVLDLFEKMMEDM
NLNEEKKAPLRNKDFTTKREMVVQYISAT


Epitope

Binds to an epitope located within the peptide sequence SLNLSEKEVLDLFEK as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL1111

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DIAPH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong staining in the trophoblast.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows absence of immunoreactivity (negative control).