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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- GTX10562 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX10562, RRID:AB_422219
- Product name
- DIAPH2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot using GeneTex Affinity Purified anti-DIAPH2 antibody (GTX10562) shows detection of a 132-kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
