TA319446

antibody from OriGene

Targeting: DIAPH2 DIA, DIA2, POF, POF2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319446

Provider

OriGene

Product name

Rabbit polyclonal anti-DIA-2 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-DIA-2 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

DIAPH2

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.4 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using Anti-DIA-2 antibody shows detection of a 132-kDa band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Validation comment

WB