PAB20307

antibody from Abnova Corporation

Targeting: DIAPH2 DIA, DIA2, POF, POF2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20307

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20307, RRID:AB_10967143

Product name

DIAPH2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DIAPH2.

Antigen sequence

GGSEEPGGGRSNKRSAGNRAANEEETKNKPKLNIQ
IKTLADDVRDRITSFRKSTVKKEKPLIQHPIDSQV
AMSEFPAAQPLYDERSLNLSEKEVLDLFEKMMEDM
NLNEEKKAPLRNKDFTTKREMVVQYISAT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DIAPH2 polyclonal antibody (Cat # PAB20307) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with DIAPH2 polyclonal antibody (Cat # PAB20307) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with DIAPH2 polyclonal antibody (Cat # PAB20307) at 1-4 ug/mL dilution shows positivity in nucleoli, cytoplasm, vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with DIAPH2 polyclonal antibody (Cat # PAB20307) shows cytoplasmic positivity in cells of seminiferus ducts at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)