PA5-52173

antibody from Invitrogen Antibodies

Targeting: DIAPH2 DIA, DIA2, POF, POF2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52173

Provider

Invitrogen Antibodies

Product name

DIAPH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GGSEEPGGGR SNKRSAGNRA ANEEETKNKP KLNIQIKTLA DDVRDRITSF RKSTVKKEKP LIQHPIDSQV AMSEFPAAQP LYDERSLNLS EKEVLDLFEK MMEDMNLNEE KKAPLRNKDF TTKREMVVQY ISAT

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DIAPH2 in Lane 1: Marker (kDa) 220, 112, 84, 47, 32, 26, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a DIAPH2 Polyclonal Antibody (Product # PA5-52173).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DIAPH2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DIAPH2 Polyclonal Antibody (Product # PA5-52173).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DIAPH2 in human cell line U-251 MG shows positivity in nucleoli, the Golgi apparatus & vesicles. Samples were probed using a DIAPH2 Polyclonal Antibody (Product # PA5-52173).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DIAPH2 in human cell line U-251 MG using a DIAPH2 Polyclonal Antibody (Product # PA5-52173) shows localization to nucleoli, endoplasmic reticulum and vesicles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DIAPH2 in human placenta and pancreas tissues using DIAPH2 Polyclonal Antibody (Product # PA5-52173). Corresponding DIAPH2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DIAPH2 in human placenta using DIAPH2 Polyclonal Antibody (Product # PA5-52173) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DIAPH2 in human pancreas using DIAPH2 Polyclonal Antibody (Product # PA5-52173) shows low expression as expected.