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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09268PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09268PU-N, RRID:AB_2035412
- Product name
- anti DIAPH2 (Isoform 2: DIA-156)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding aa 1085-1101 of Human DIA-2
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.4 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using Affinity Purified anti-DIA-2 antibody shows detection of a 132-kDa band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
