66269-1-IG

antibody from Invitrogen Antibodies

Targeting: L2HGDH C14orf160, FLJ12618

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66269-1-IG

Provider

Invitrogen Antibodies

Product name

L2HGDH Monoclonal Antibody (3D11C2)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MVPALRYLV GACGRARGRF AGGSPGACGF ASGRPRPLCG GSRSASTSSF DIVIVGGGIV GLASARALIL RHPSLSIGVL EKEKDLAVHQ TGHNSGVIHS GIYYKPESLK AKLCVQGAAL LYEYCQQKGI SYKQCGKLIV AVEQEEIPRL QALYEKGLQN GVPGLRLIQQ EDIKKKEPYC RGLMAIDCPH TGIVDYRQVA LSFAQDFQEA GGSVLTNFEV KGIEMAKESP SRSIDGMQYP IVIKNTKGEE IRCQYVVTCA GLYSDRISEL SGCTPDPRIV PFRGDYLLLK PEKCYLVKGN IYPVPDSRFP FLGVHFTPRM DGSIWLGPNA VLAFKREGYR PFDFSATDVM DI (1-351 aa encoded by BC006117)

Reactivity

Human, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

3D11C2

Vial size

150 µL

Concentration

1.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Pig brain tissue were subjected to SDS PAGE followed by western blot with 66269-1-IG ( L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66269-1-IG ( L2HGDH Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66269-1-IG ( L2HGDH Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (Product # 66269-1-IG, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).