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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP1-00178 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00178, RRID:AB_1503017
- Product name
- Goat Polyclonal DIO2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb.
Prolonged inorganic arsenic exposure via drinking water impairs brown adipose tissue function in mice.
Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes.
Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage.
Contributions of HIV infection in the hypothalamus and substance abuse/use to HPT dysregulation.
Ala92 type 2 deiodinase allele increases risk for the development of hypertension.
Myers DA, Singleton K, Hyatt K, Kaushal KM, Ducsay CA
Adipocyte 2020 Dec;9(1):223-233
Adipocyte 2020 Dec;9(1):223-233
Prolonged inorganic arsenic exposure via drinking water impairs brown adipose tissue function in mice.
Zuo Z, Liu Z, Gao T, Yin Y, Wang Z, Hou Y, Fu J, Liu S, Wang H, Xu Y, Pi J
The Science of the total environment 2019 Jun 10;668:310-317
The Science of the total environment 2019 Jun 10;668:310-317
Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes.
Kishida T, Ejima A, Yamamoto K, Tanaka S, Yamamoto T, Mazda O
Stem cell reports 2015 Oct 13;5(4):569-81
Stem cell reports 2015 Oct 13;5(4):569-81
Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage.
Vose LR, Vinukonda G, Jo S, Miry O, Diamond D, Korumilli R, Arshad A, Zia MT, Hu F, Kayton RJ, La Gamma EF, Bansal R, Bianco AC, Ballabh P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Oct 30;33(44):17232-46
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Oct 30;33(44):17232-46
Contributions of HIV infection in the hypothalamus and substance abuse/use to HPT dysregulation.
Langford D, Baron D, Joy J, Del Valle L, Shack J
Psychoneuroendocrinology 2011 Jun;36(5):710-9
Psychoneuroendocrinology 2011 Jun;36(5):710-9
Ala92 type 2 deiodinase allele increases risk for the development of hypertension.
Gumieniak O, Perlstein TS, Williams JS, Hopkins PN, Brown NJ, Raby BA, Williams GH
Hypertension (Dallas, Tex. : 1979) 2007 Mar;49(3):461-6
Hypertension (Dallas, Tex. : 1979) 2007 Mar;49(3):461-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DIO2 Antibody [NBP1-00178] - Staining of paraffin embedded Human Small Intestine.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody
