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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-49631 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIO2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with pig based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DIO2 in MCF-7 cell line lysates (35 µg/lane) using DIO2 Antibody (Center) (Product # PA5-49631).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DIO2 in human heart and human skeletal muscle tissue (left to right). Samples were probed with a DIO2 polyclonal antibody (Product # PA5-49631) diluted at 1:1000 at each lane. A goat rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 µg per lane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DIO2 in formalin fixed and paraffin embedded mouse brain. Samples stained using a DIO2 Antibody (Center) (Product # PA5-49631), followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of DIO2 in MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). Samples were probed with a DIO2 Antibody (Center) (Product # PA5-49631). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
