PA5-22034

antibody from Invitrogen Antibodies

Targeting: LRPPRC GP130, LRP130, LSFC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22034

Provider

Invitrogen Antibodies

Product name

LRP130 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, HeLa, mouse brain. Predicted reactivity: Mouse (84%), Rat (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.36 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Key J, Torres-Odio S, Bach NC, Gispert S, Koepf G, Reichlmeir M, West AP, Prokisch H, Freisinger P, Newman WG, Shalev S, Sieber SA, Wittig I, Auburger G
Cells 2021 Nov 29;10(12)

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Identification of host proteins differentially associated with HIV-1 RNA splice variants.

Knoener R, Evans E 3rd, Becker JT, Scalf M, Benner B, Sherer NM, Smith LM
eLife 2021 Feb 25;10

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Lipidomics unveils lipid dyshomeostasis and low circulating plasmalogens as biomarkers in a monogenic mitochondrial disorder.

Ruiz M, Cuillerier A, Daneault C, Deschênes S, Frayne IR, Bouchard B, Forest A, Legault JT, LSFC Consortium, Vaz FM, Rioux JD, Burelle Y, Des Rosiers C
JCI insight 2019 Jul 25;4(14)

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The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.

Hedgepeth SC, Garcia MI, Wagner LE 2nd, Rodriguez AM, Chintapalli SV, Snyder RR, Hankins GD, Henderson BR, Brodie KM, Yule DI, van Rossum DB, Boehning D
The Journal of biological chemistry 2015 Mar 13;290(11):7304-13

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LRP130 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a LRP130 polyclonal antibody (Product # PA5-22034) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of LRP130 was performed by separating 50 µg of mouse tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LRP130 Polyclonal Antibody (Product # PA5-22034) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using LRP130 Polyclonal Antibody (Product # PA5-22034). Sample (30 µg of whole cell lysate). A: H1299. B: HeLa.5% SDS PAGE. LRP130 Polyclonal Antibody (Product # PA5-22034) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of LRP130 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 Polyclonal Antibody (Product # PA5-22034) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of LRP130 was performed in paraffin-embedded human colon cancer tissue using LRP130 Polyclonal Antibody (Product # PA5-22034) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 (Product # PA5-22034) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.