A305-824A-T

antibody from Invitrogen Antibodies

Targeting: PPP1R21 CCDC128, FLJ16566, KLRAQ1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-824A-T

Provider

Invitrogen Antibodies

Product name

PPP1R21 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PPP1R21 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP1R21 antibody A305-824A-M (lot A305-824A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PPP1R21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP1R21 antibody (Product # A305-824A-T; lot A305-824A-T-1) used for IP at 100 µL per reaction. PPP1R21 was also immunoprecipitated by rabbit anti-PPP1R21 antibody (Product # A305-825A-T). For blotting immunoprecipitated PPP1R21, Product # A305-824A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.