MA5-47719

antibody from Invitrogen Antibodies

Targeting: CCDC88A APE, FLJ10392, GIV, GRDN, HkRP1, KIAA1212

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-47719

Provider

Invitrogen Antibodies

Product name

Girdin Monoclonal Antibody (M012)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

M012

Vial size

100 µL

Concentration

~0.25 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Girdin in human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse Girdin monoclonal antibody (Product # MA5-47719) (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr1764), phospho-specific (lanes 3 & 4).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse Girdin monoclonal antibody (Product # MA5-47719). The antibody was detected using goat anti-mouse DyLight® 594.