Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 14132-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14132-1-AP, RRID:AB_2110155
- Product name
- GFPT1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GFPT1 antibody (Cat. #14132-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate.
[WITHDRAWN] Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction.
The UDPase ENTPD5 regulates ER stress-associated renal injury by mediating protein N-glycosylation.
Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P.
ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes Nephropathy.
GFPT1-Associated Congenital Myasthenic Syndrome Mimicking a Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology Solved by Next-Generation-Sequencing.
GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.
O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.
GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.
DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation.
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.
Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle.
Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.
Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.
Hydrogen sulfide accumulates LDL receptor precursor via downregulating PCSK9 in HepG2 cells.
Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.
GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.
Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.
MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
A Genetic Model to Study Increased Hexosamine Biosynthetic Flux.
Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.
A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome.
Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.
Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.
Zhou P, Chang WY, Gong DA, Xia J, Chen W, Huang LY, Liu R, Liu Y, Chen C, Wang K, Tang N, Huang AL
Cell metabolism 2023 Nov 7;35(11):1961-1975.e6
Cell metabolism 2023 Nov 7;35(11):1961-1975.e6
[WITHDRAWN] Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction.
Tatekoshi Y, Shapiro JS, Liu M, Bianco GM, Tatekoshi A, De Jesus A, Koleini N, Wasserstrom JA, Dillmann WH, Weinberg SE, Ardehali H
Research square 2023 Jan 25;
Research square 2023 Jan 25;
The UDPase ENTPD5 regulates ER stress-associated renal injury by mediating protein N-glycosylation.
Xu L, Zhou Y, Wang G, Bo L, Jin B, Dai L, Lu Q, Cai X, Hu L, Liu L, Wu Y, Chang X, Huang Y, Song L, Zhang T, Wang Y, Xiao Y, Zhang F, Liu L, Shi M, Wang T, Guo B
Cell death & disease 2023 Feb 27;14(2):166
Cell death & disease 2023 Feb 27;14(2):166
Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P.
Ai YL, Wang WJ, Liu FJ, Fang W, Chen HZ, Wu LZ, Hong X, Zhu Y, Zhang CX, Liu LY, Hong WB, Zhou B, Chen QT, Wu Q
Cell research 2023 Dec;33(12):904-922
Cell research 2023 Dec;33(12):904-922
ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes Nephropathy.
Song S, Hu T, Shi X, Jin Y, Liu S, Li X, Zou W, Wang C
International journal of molecular sciences 2023 Dec 18;24(24)
International journal of molecular sciences 2023 Dec 18;24(24)
GFPT1-Associated Congenital Myasthenic Syndrome Mimicking a Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology Solved by Next-Generation-Sequencing.
Mensch A, Cordts I, Scholle L, Joshi PR, Kleeberg K, Emmer A, Beck-Woedl S, Park J, Haack TB, Stoltenburg-Didinger G, Zierz S, Deschauer M
Journal of neuromuscular diseases 2022;9(4):533-541
Journal of neuromuscular diseases 2022;9(4):533-541
GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.
Li D, Guan M, Cao X, Zha ZQ, Zhang P, Xiang H, Zhou Y, Peng Q, Xu Z, Lu L, Liu G
Carcinogenesis 2022 Nov 23;43(10):969-979
Carcinogenesis 2022 Nov 23;43(10):969-979
O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.
Zhang H, Qi J, Pei J, Zhang M, Shang Y, Li Z, Wang Y, Guo J, Sun K, Fan J, Sui L, Xu Y, Kong L, Kong Y
Journal of advanced research 2022 Mar;37:119-131
Journal of advanced research 2022 Mar;37:119-131
GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.
Wei S, Zhao Q, Zheng K, Liu P, Sha N, Li Y, Ma C, Li J, Zhuo L, Liu G, Liang W, Jiang Y, Chen T, Zhong N
Cell discovery 2022 Aug 9;8(1):77
Cell discovery 2022 Aug 9;8(1):77
DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation.
Song T, Zou Q, Yan Y, Lv S, Li N, Zhao X, Ma X, Liu H, Tang B, Sun L
Cell reports 2021 Sep 21;36(12):109739
Cell reports 2021 Sep 21;36(12):109739
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.
Oliva Chávez AS, Wang X, Marnin L, Archer NK, Hammond HL, Carroll EEM, Shaw DK, Tully BG, Buskirk AD, Ford SL, Butler LR, Shahi P, Morozova K, Clement CC, Lawres L, Neal AJO, Mamoun CB, Mason KL, Hobbs BE, Scoles GA, Barry EM, Sonenshine DE, Pal U, Valenzuela JG, Sztein MB, Pasetti MF, Levin ML, Kotsyfakis M, Jay SM, Huntley JF, Miller LS, Santambrogio L, Pedra JHF
Nature communications 2021 Jun 17;12(1):3696
Nature communications 2021 Jun 17;12(1):3696
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.
Ou W, Liang Y, Qin Y, Wu W, Xie M, Zhang Y, Zhang Y, Ji L, Yu H, Li T
Redox biology 2021 Jul;43:101994
Redox biology 2021 Jul;43:101994
Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle.
Weyrauch LA, McMillin SL, Witczak CA
International journal of molecular sciences 2020 Jul 1;21(13)
International journal of molecular sciences 2020 Jul 1;21(13)
Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.
Paredes F, Williams HC, Quintana RA, San Martin A
Circulation research 2020 Jan 3;126(1):41-56
Circulation research 2020 Jan 3;126(1):41-56
Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.
Sharma NS, Gupta VK, Garrido VT, Hadad R, Durden BC, Kesh K, Giri B, Ferrantella A, Dudeja V, Saluja A, Banerjee S
The Journal of clinical investigation 2020 Jan 2;130(1):451-465
The Journal of clinical investigation 2020 Jan 2;130(1):451-465
Hydrogen sulfide accumulates LDL receptor precursor via downregulating PCSK9 in HepG2 cells.
Huang Y, Ning K, Li WW, Lin G, Hou CL, Wang MJ, Zhu YC
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1082-C1096
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1082-C1096
Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.
Qin L, Wang J, Zhao R, Zhang X, Mei Y
Journal of agricultural and food chemistry 2019 Dec 26;67(51):14074-14085
Journal of agricultural and food chemistry 2019 Dec 26;67(51):14074-14085
GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.
Issop Y, Hathazi D, Khan MM, Rudolf R, Weis J, Spendiff S, Slater CR, Roos A, Lochmüller H
Human molecular genetics 2018 Sep 15;27(18):3218-3232
Human molecular genetics 2018 Sep 15;27(18):3218-3232
Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.
Dai W, Dierschke SK, Toro AL, Dennis MD
Biochimica et biophysica acta. Molecular basis of disease 2018 Dec;1864(12):3568-3576
Biochimica et biophysica acta. Molecular basis of disease 2018 Dec;1864(12):3568-3576
MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
Shtraizent N, DeRossi C, Nayar S, Sachidanandam R, Katz LS, Prince A, Koh AP, Vincek A, Hadas Y, Hoshida Y, Scott DK, Eliyahu E, Freeze HH, Sadler KC, Chu J
eLife 2017 Jun 23;6
eLife 2017 Jun 23;6
A Genetic Model to Study Increased Hexosamine Biosynthetic Flux.
Hugo SE, Schlegel A
Endocrinology 2017 Aug 1;158(8):2420-2426
Endocrinology 2017 Aug 1;158(8):2420-2426
Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.
Oikari S, Makkonen K, Deen AJ, Tyni I, Kärnä R, Tammi RH, Tammi MI
Glycobiology 2016 Jul;26(7):710-22
Glycobiology 2016 Jul;26(7):710-22
A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome.
Dusl M, Senderek J, Müller JS, Vogel JG, Pertl A, Stucka R, Lochmüller H, David R, Abicht A
Human molecular genetics 2015 Jun 15;24(12):3418-26
Human molecular genetics 2015 Jun 15;24(12):3418-26
Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.
Ganguly R, Sahu S, Chavez RJ, Raman P
American journal of physiology. Cell physiology 2015 Jan 15;308(2):C111-22
American journal of physiology. Cell physiology 2015 Jan 15;308(2):C111-22
Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.
Senderek J, Müller JS, Dusl M, Strom TM, Guergueltcheva V, Diepolder I, Laval SH, Maxwell S, Cossins J, Krause S, Muelas N, Vilchez JJ, Colomer J, Mallebrera CJ, Nascimento A, Nafissi S, Kariminejad A, Nilipour Y, Bozorgmehr B, Najmabadi H, Rodolico C, Sieb JP, Steinlein OK, Schlotter B, Schoser B, Kirschner J, Herrmann R, Voit T, Oldfors A, Lindbergh C, Urtizberea A, von der Hagen M, Hübner A, Palace J, Bushby K, Straub V, Beeson D, Abicht A, Lochmüller H
American journal of human genetics 2011 Feb 11;88(2):162-72
American journal of human genetics 2011 Feb 11;88(2):162-72
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP(GFPT1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 14132-1-AP(GFPT1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 14132-1-AP(GFPT1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue