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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX102919 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102919, RRID:AB_10630636
- Product name
- Nuclear Matrix Protein p84 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Protein Cofactors Are Essential for High-Affinity DNA Binding by the Nuclear Factor κB RelA Subunit.
MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERα)-positive mammary adenocarcinomas.
The THO ribonucleoprotein complex is required for stem cell homeostasis in the adult mouse small intestine.
Mulero MC, Shahabi S, Ko MS, Schiffer JM, Huang DB, Wang VY, Amaro RE, Huxford T, Ghosh G
Biochemistry 2018 May 22;57(20):2943-2957
Biochemistry 2018 May 22;57(20):2943-2957
MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERα)-positive mammary adenocarcinomas.
Mukherjee M, Ge G, Zhang N, Edwards DG, Sumazin P, Sharan SK, Rao PH, Medina D, Pati D
Oncogene 2014 Nov 27;33(48):5511-5522
Oncogene 2014 Nov 27;33(48):5511-5522
The THO ribonucleoprotein complex is required for stem cell homeostasis in the adult mouse small intestine.
Pitzonka L, Wang X, Ullas S, Wolff DW, Wang Y, Goodrich DW
Molecular and cellular biology 2013 Sep;33(17):3505-14
Molecular and cellular biology 2013 Sep;33(17):3505-14
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 7.5% SDS PAGE Tho1 antibody GTX102919 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nuclear Matrix Protein p84 antibody [C1C3] detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Nuclear Matrix Protein p84 antibody [C1C3] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 £gg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 £gg of Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] (GTX102919) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Nuclear Matrix Protein p84 (GTX102919) antibody at 1:500 dilution.
Supportive validation
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- GeneTex (provider)
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- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-Nuclear Matrix Protein p84 antibody. The precipitated DNA was detected by PCR with primer set targeting to calreticulin.
