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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-27816 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nuclear Matrix Protein p84 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat-2, THOC1 shRNA-transfected 293T.
- Concentration
- 1 mg/mL
Submitted references ITGBL1 promotes EMT, invasion and migration by activating NF-κB signaling pathway in prostate cancer.
Downregulation of miR‑505‑3p predicts poor bone metastasis‑free survival in prostate cancer.
N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway.
Downregulation of miR‑19a‑3p promotes invasion, migration and bone metastasis via activating TGF‑β signaling in prostate cancer.
Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway.
Downregulation of miR-141-3p promotes bone metastasis via activating NF-κB signaling in prostate cancer.
Li W, Li S, Yang J, Cui C, Yu M, Zhang Y
OncoTargets and therapy 2019;12:3753-3763
OncoTargets and therapy 2019;12:3753-3763
Downregulation of miR‑505‑3p predicts poor bone metastasis‑free survival in prostate cancer.
Tang Y, Wu B, Huang S, Peng X, Li X, Huang X, Zhou W, Xie P, He P
Oncology reports 2019 Jan;41(1):57-66
Oncology reports 2019 Jan;41(1):57-66
N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway.
Yin Y, Xu L, Chang Y, Zeng T, Chen X, Wang A, Groth J, Foo WC, Liang C, Hu H, Huang J
Molecular cancer 2019 Jan 18;18(1):11
Molecular cancer 2019 Jan 18;18(1):11
Downregulation of miR‑19a‑3p promotes invasion, migration and bone metastasis via activating TGF‑β signaling in prostate cancer.
Wa Q, Li L, Lin H, Peng X, Ren D, Huang Y, He P, Huang S
Oncology reports 2018 Jan;39(1):81-90
Oncology reports 2018 Jan;39(1):81-90
Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway.
Ren D, Yang Q, Dai Y, Guo W, Du H, Song L, Peng X
Molecular cancer 2017 Jul 10;16(1):117
Molecular cancer 2017 Jul 10;16(1):117
Downregulation of miR-141-3p promotes bone metastasis via activating NF-κB signaling in prostate cancer.
Huang S, Wa Q, Pan J, Peng X, Ren D, Huang Y, Chen X, Tang Y
Journal of experimental & clinical cancer research : CR 2017 Dec 4;36(1):173
Journal of experimental & clinical cancer research : CR 2017 Dec 4;36(1):173
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nuclear Matrix Protein p84 using 30 µg of A549 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Nuclear Matrix Protein p84 polyclonal antibody (Product # PA5-27816) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nuclear Matrix Protein p84 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nuclear Matrix Protein p84 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Nuclear Matrix Protein p84 Polyclonal Antibody detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Nuclear Matrix Protein p84 (Product # PA5-27816) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816). The precipitated DNA was detected by PCR with primer set targeting to calreticulin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Nuclear Matrix Protein p84 Polyclonal Antibody immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 µg of Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
