12474-1-AP

antibody from Invitrogen Antibodies

Targeting: SEMA4F SEMAM, SEMAW

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12474-1-AP

Provider

Invitrogen Antibodies

Product name

SEMA4F Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LVTTDTAYL RVVAHRVTSL SGKEYDVLYL GTEDGHLHRA VRIGAQLSVL EDLALFPEPQ PVENMKLYHS WLLVGSRTEV TQVNTTNCGR LQSCSECILA QDPVCAWSFR LDECVAHAGE HRGLVQDIES ADVSSLCPKE PGERPVVFEV PVATAAHVVL PCSPSSAWAS CVWHQPSGVT ALTPRRDGLE VVVTPGAMGA YACECQEGGA AHVVAAYSLV WGSQRDAPSR AHTVGAGLAG FFLGILAASL TLILIGRRQQ RRRQRELLAR DKVGLDLGAP PSGTTSYSQD PPSPSPEDER LPLALAKRGS GFGGFSPPFL LDPCPSPAHI RLTGAPLATC DETSI (272-615 aa encoded by BC018361)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SEMA4F (IP:12474-1-AP, 4ug; Detection:12474-1-AP 1:500) with HepG2 cells lysate 3600ug.