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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- MAB2098 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Reg2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Reg2 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Reg1, recombinant rat (rr) Reg2, rmReg3A, recombinant human Reg4, or rrReg4.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Adjuvant immunotherapy increases beta cell regenerative factor Reg2 in the pancreas of diabetic mice.
Huszarik K, Wright B, Keller C, Nikoopour E, Krougly O, Lee-Chan E, Qin HY, Cameron MJ, Gurr WK, Hill DJ, Sherwin RS, Kelvin DJ, Singh B
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5120-9
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5120-9
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse Reg2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line, mouse small intestine and pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg2 Monoclonal Antibody (Catalog # MAB2098) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
