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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX109215 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109215, RRID:AB_1952099
- Product name
- SUCLG1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Screen for abnormal mitochondrial phenotypes in mouse embryonic stem cells identifies a model for succinyl-CoA ligase deficiency and mtDNA depletion.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
Screen for abnormal mitochondrial phenotypes in mouse embryonic stem cells identifies a model for succinyl-CoA ligase deficiency and mtDNA depletion.
Donti TR, Stromberger C, Ge M, Eldin KW, Craigen WJ, Graham BH
Disease models & mechanisms 2014 Feb;7(2):271-80
Disease models & mechanisms 2014 Feb;7(2):271-80
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- SUCLG1 antibody detects SUCLG1 protein by western blot analysis.A.50 ?g rat brain lysate/extract 10% SDS-PAGESUCLG1 antibody (GTX109215) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: A43110% SDS PAGEGTX109215 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX109215 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: SUCLG1 protein stained by SUCLG1 antibody (GTX109215) diluted at 1:500.Blue: Hoechst 33342 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SUCLG1(GTX109215) antibody at 1:100 dilution.
