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LearnPA5-38051
antibody from Invitrogen Antibodies
Targeting: IL1F10
FKSG75, IL-1F10, IL-1HY2, IL1-theta, MGC11983, MGC119832, MGC119833
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-38051 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1F10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A recommended positive control is human liver tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Deficiency in the phosphatase PHLPP1 suppresses osteoclast-mediated bone resorption and enhances bone formation in mice.
Mattson AM, Begun DL, Molstad DHH, Meyer MA, Oursler MJ, Westendorf JJ, Bradley EW
The Journal of biological chemistry 2019 Aug 2;294(31):11772-11784
The Journal of biological chemistry 2019 Aug 2;294(31):11772-11784
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1F10 in human liver tissue lysate using an IL1F10 polyclonal antibody (Product # PA5-38051) at a dilution of 1 µg/mL in (A) the absence and (B) the presence of a blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Liver Tissue Lysate in the (A) absence or (B) presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: IL1F10 Polyclonal Antibody (Product # PA5-38051) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-1F10 recombinant protein per lane. Antibodies: IL1F10 Polyclonal Antibody (Product # PA5-38051) (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human spleen tissue using IL1F10 Polyclonal Antibody (Product # PA5-38051) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
