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antibody from Invitrogen Antibodies
Targeting: IL1F10
FKSG75, IL-1F10, IL-1HY2, IL1-theta, MGC11983, MGC119832, MGC119833
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- 14-7385-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-38 Monoclonal Antibody (H127C), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody H127C recognizes human IL-38. IL-38 is a member of the Interleukin-1 family (IL-1F) and shares homology with IL-1R antagonist (IL-1Ra) and IL-36R antagonist (IL-36Ra). IL-38 is expressed mostly in the skin and proliferating B cells of the tonsil and is also present at low levels in the heart and placenta. IL-38 functions as an anti-inflammatory cytokine that inhibits IL-36 by binding to IL-36R and also inhibits the production of IL-17 and IL-22. Gene polymorphisms of IL-38 are associated with psoriatic arthritis, ankylosing spondylitis, and heart disease. Applications Reported: This H127C antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, ELISA, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This H127C antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- H127C
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
Cloning and characterization of IL-1HY2, a novel interleukin-1 family member.
van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, van der Meer JW, Hao R, Kalabokis V, Dinarello CA
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):3001-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):3001-5
Cloning and characterization of IL-1HY2, a novel interleukin-1 family member.
Lin H, Ho AS, Haley-Vicente D, Zhang J, Bernal-Fussell J, Pace AM, Hansen D, Schweighofer K, Mize NK, Ford JE
The Journal of biological chemistry 2001 Jun 8;276(23):20597-602
The Journal of biological chemistry 2001 Jun 8;276(23):20597-602
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human skin tissue using 0.1 µg/mL of Mouse IgG2b K Isotype Control Purified (left) or 0.1 µg/mL of Anti-Human IL-38 Purified (right) followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
