Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [2]
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Validation data
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- Product number
- NBP1-50029 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50029, RRID:AB_10012219
- Product name
- Rabbit Polyclonal ANKRD27 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: ANKRD27 Antibody [NBP1-50029] - Detection of Human Varp by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes a downstream epitope. For blotting immunoprecipitated Varp was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ANKRD27 Antibody [NBP1-50029] - Detection of human Varp by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Varp antibody NBP1-50029 used for IP at 3 ug per reaction. Varp was also immunoprecipitated by rabbit anti-Varp antibody NBP1-50030. Detection:Chemiluminescence with an exposure time of 3 minutes.