NBP2-59321

antibody from Novus Biologicals

Targeting: SPTBN4 KIAA1642, SPTBN3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-59321

Provider

Novus Biologicals

Product name

Mouse Monoclonal SPTBN4 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects >200kDa. Does not cross react with other Beta-spectrins

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SPTBN4 Antibody (S393-2) [NBP2-59321] - Analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 ug protein. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.