MA5-27608

antibody from Invitrogen Antibodies

Targeting: SPTBN4 KIAA1642, SPTBN3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27608

Provider

Invitrogen Antibodies

Product name

Spectrin beta-4 Monoclonal Antibody (S393-2)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S393-2

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Spectrin beta-4 in COS-Beta-4-Spectrin-His with 15 µg of sample. The sample was incubated with Spectrin beta-4 monoclonal antibody (Product # MA5-27608) using a dilution of 1:1000 (16 hours at 4°C), followed by Goat Anti-Mouse (1 hour at RT) at a dilution of 1:200 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: COS-Beta-4-Spectrin-His.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Spectrin beta-4 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Spectrin beta-4 monoclonal antibody (Product # MA5-27608) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) beta 4 Spectrin Antibody, D) Merged image. Magnification: 60x.