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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-50668 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Glucagon Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total GCGR / Glucagon Receptor protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.7 mg/mL
- Storage
- -20°C
Submitted references Glucagon signaling via supraphysiologic GCGR can reduce cell viability without stimulating gluconeogenic gene expression in liver cancer cells.
Godfrey J, Riscal R, Skuli N, Simon MC
Cancer & metabolism 2022 Feb 5;10(1):4
Cancer & metabolism 2022 Feb 5;10(1):4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCGR was performed by loading 293T cell lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a GCGR antibody (Product # PA5-50668) at a 1/200 dilution for 30 seconds, followed by a secondary antibody at 1/8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Glucagon Receptor in paraffin embedded Human thyroid cancer tissue using Glucagon Receptor Polyclonal Antibody (Product # PA5-50668) at a 1:40 dilution.
