21399-1-AP

antibody from Invitrogen Antibodies

Targeting: U2SURP fSAPa, SR140

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21399-1-AP

Provider

Invitrogen Antibodies

Product name

SR140 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KETEDVPDD LDGAPIEEEL DGAPLEDVDG IPIDATPIDD LDGVPIKSLD DDLDGVPLDA TEDSKKNEPI FKVAPSKWEA VDESELEAQA VTTSKWELFD QHEESEEEEN QNQEEESEDE EDTQSSKSEE HHLYSNPIKE EMTESKFSKY SEMSEEKRAK LREIELKVMK FQDELESGKR PKKPGQSFQE QVEHYRDKLL QREKEKELER ERERDKKDKE KLESRSKDKK EKDECTPTRK ERKRRHSTSP SPSRSSSGRR VKSPSPKSER SERSERSHKE SSRSRSSHKD SPRDVSKKAK RSPSGSRTPK RSRRSRSRSP KKSGKKSRSQ SRSPHRSHKK SKKNKH (685-1029 aa encoded by BC111692 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.44 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 293 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens).