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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-12787 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PCF11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCF11 Antibody [NBP2-12787] - Detection of human PCF11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PCF11 antibody NBP2-12787 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PCF11 was also immunoprecipitated by rabbit anti-PCF11 antibody NBP2-12786, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PCF11 Antibody [NBP2-12787] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PCF11 antibody NBP2-12786 used for IP at 6 ug/mg lysate. PCF11 was also immunoprecipitated by rabbit anti-PCF11 antibody NBP2-12787, which recognizes a downstream epitope. For blotting immunoprecipitated PCF11, NBP2-12787 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
