PAB27892

antibody from Abnova Corporation

Targeting: FAM217B C20orf177, dJ551D2.5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27892

Provider

Abnova Corporation

Product name

FAM217B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FAM217B.

Antigen sequence

SSKSTKLQRWDLSGSGSSSKVETSGHIRVPKQAAV
ILDSADSCKASKTQAHAHPRKKGKAESCGHATVSS
EKKLKTNGVKQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with FAM217B polyclonal antibody (Cat # PAB27892) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with FAM217B polyclonal antibody (Cat # PAB27892) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder with FAM217B polyclonal antibody (Cat # PAB27892) shows strong nucleaar positivity in urothelial cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)