HPA035693

antibody from Atlas Antibodies

Targeting: AP5Z1 KIAA0415, SPG48, zeta

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA035693

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA035693, RRID:AB_10960687

Product name

Anti-AP5Z1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSE
QEGSTLSVISATSSAGRLLPPRERLREVAFEYCQR
LIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQ
DPSFLYRSLS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease.

Hirst J, Edgar JR, Esteves T, Darios F, Madeo M, Chang J, Roda RH, Dürr A, Anheim M, Gellera C, Li J, Züchner S, Mariotti C, Stevanin G, Blackstone C, Kruer MC, Robinson MS
Human molecular genetics 2015 Sep 1;24(17):4984-96

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-AP5Z1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.

Sample type

HUMAN