Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 66533-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AP5Z1/SPG48 Monoclonal Antibody (1G1D7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: TLDTDDRSE QEGSTLSVIS ATSSAGRLLP PRERLREVAF EYCQRLIEQS NRRALRKGDS DLQKACLVEA VLVLDVLCRQ DPSFLYRSLS CLKALHGRVR GDPASV (260-364 aa encoded by BC037399)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1G1D7
- Vial size
- 150 µL
- Concentration
- 0.7 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-IG (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-IG (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HEK-293 cells using 66533-1-IG (AP5Z1/SPG48 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IGG (H+L).