LS-B32

antibody from LSBio

Targeting: HP

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B32

Provider

LSBio

Product name

PathPlus™ HP / Haptoglobin Antibody LS-B32

Antibody type

Polyclonal

Description

Affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

References

Submitted references

The extracellular chaperone haptoglobin prevents serum fatty acid-promoted amyloid fibril formation of β2-microglobulin, resistance to lysosomal degradation, and cytotoxicity.

Sultan A, Raman B, Rao CM, Tangirala R
The Journal of biological chemistry 2013 Nov 8;288(45):32326-32342

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA results of purified Rabbit anti-Human Haptoglobin Antibody tested against immunizing antigen. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA results of purified Rabbit anti-Human Haptoglobin Antibody tested against immunizing antigen. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA results of purified Rabbit anti-Human Haptoglobin Antibody tested against immunizing antigen. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-Haptoglobin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 31 ug/ml.