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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-79391 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Haptoglobin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Hypertransaminasemia and liver fibrosis associated with haptoglobin retention and anhaptoglobinemia in a paediatric patient.
Gunzer S, Kraus A, Buchroth I, Grüneberg M, Westermann C, Biskup S, Reunert J, Grünewald I, Marquardt T
Liver international : official journal of the International Association for the Study of the Liver 2021 Oct;41(10):2427-2432
Liver international : official journal of the International Association for the Study of the Liver 2021 Oct;41(10):2427-2432
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Haptoglobin in Lane 1: HeLa whole cell lysate, Lane 2: SMMC whole cell lysate, Lane 3: HEPA whole cell lysate, Lane 4: HEPG2 whole cell lysate using 40-50 µg per well. Sample was incubated with Haptoglobin (Product # PA5-79391) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Haptoglobin in recombinant human Haptoglobin protein using 0.5 ng per well. Sample was incubated with Haptoglobin polyclonal antibody (Product # PA5-79391) at a dilution of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Haptoglobin on paraffin-embedded human placenta tissue. Sample was incubated with Haptoglobin polyclonal antibody (Product# PA5-79391).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 FIGURE Immunohistochemistry for immunodetection of Hp in liver biopsy tissue and Western Blot. 2.1 Immunohistochemistry for immunodetection of Hp in liver biopsy tissue. A, Control tissue stained with the monoclonal anti-haptoglobin antibody. Positive staining reaction shows (brown) presence of haptoglobin in the cytoplasm of hepatocytes. Original magnification 400x. B, The monoclonal antibody is not able to detect haptoglobin in the patient-derived tissue. Original magnification 400x. C, Control tissue stained with the polyclonal anti-haptoglobin antibody. Positive staining reaction shows presence of haptoglobin in the hepatocytes. Original magnification 400x. D, Patient`s tissue stained with the polyclonal anti-haptoglobin antibody. Positive staining reaction shows presence of haptoglobin in the hepatocytes. Original magnification 400x. 2.2 Western Blot detection of Haptoglobin in blood serum. C1, C2 = control serum of healthy individuals (m/f) show normal level of haptoglobin. F = patient`s father, shows low level of haptoglobin. P = patient shows no serum haptoglobin. alpha-chain (alpha-2) = 18 kDa. beta-chain = 40 kDa
