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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM1031a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1031a, RRID:AB_1968384
- Product name
- TBB5 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of anti-TBB5 Antibody (Cat. #AM1031a) in Y79,CEM,293 cell line and mouse brain tissue lysates (35ug/lane). TBB5(arrow) was detected using the purified Mab.(1ug/ml)"
- Primary Ab dilution
- 1:100~500
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent analysis of A549 cells, using TBB5 Antibody (Cat. #AM1031a). AM1031a was diluted at 1ÿ25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red)."
- Primary Ab dilution
- 1:25
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
