GTX101279

antibody from GeneTex

Targeting: TUBB M40, MGC16435, OK/SW-cl.56, Tubb5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX101279

Provider

GeneTex

Proper citation

GeneTex Cat#GTX101279, RRID:AB_1952434

Product name

beta Tubulin antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Hamster

Host

Rabbit

References

Submitted references

ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.

Pai VC, Hsu CC, Chan TS, Liao WY, Chuu CP, Chen WY, Li CR, Lin CY, Huang SP, Chen LT, Tsai KK
Oncogene 2019 Feb;38(8):1340-1353

---

Abnormal Expression of PICT-1 and Its Codon 389 Polymorphism Is a Risk Factor for Human Endometrial Cancer.

Yoshimoto M, Tokuda A, Nishiwaki K, Sengoku K, Yaginuma Y
Oncology 2018;95(1):43-51

---

Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.

Wakae K, Nishiyama T, Kondo S, Izuka T, Que L, Chen C, Kase K, Kitamura K, Mohiuddin M, Wang Z, Ahasan MM, Nakamura M, Fujiwara H, Yoshizaki T, Hosomochi K, Tajima A, Nakahara T, Kiyono T, Muramatsu M
Scientific reports 2018 Jun 27;8(1):9745

---

Osteoblast-Secreted Factors Mediate Dormancy of Metastatic Prostate Cancer in the Bone via Activation of the TGFβRIII-p38MAPK-pS249/T252RB Pathway.

Yu-Lee LY, Yu G, Lee YC, Lin SC, Pan J, Pan T, Yu KJ, Liu B, Creighton CJ, Rodriguez-Canales J, Villalobos PA, Wistuba II, de Nadal E, Posas F, Gallick GE, Lin SH
Cancer research 2018 Jun 1;78(11):2911-2924

---

Quercetin improves postpartum hypogalactia in milk-deficient mice via stimulating prolactin production in pituitary gland.

Lin M, Wang N, Yao B, Zhong Y, Lin Y, You T
Phytotherapy research : PTR 2018 Aug;32(8):1511-1520

---

SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules.

Wang F, Zheng L, Yi Y, Yang Z, Qiu Q, Wang X, Yan W, Bai P, Yang J, Li D, Pei H, Niu T, Ye H, Nie C, Hu Y, Yang S, Wei Y, Chen L
Molecular cancer therapeutics 2018 Apr;17(4):763-775

---

miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.

Her LS, Mao SH, Chang CY, Cheng PH, Chang YF, Yang HI, Chen CM, Yang SH
Theranostics 2017;7(9):2452-2462

---

The protein interacting with carboxyl terminus-1 codon 389 polymorphism impairs protein interacting with carboxyl terminus-1 function and is a risk factor for uterine cervical cancer.

Yoshimoto M, Tokuda A, Nishiwaki K, Sengoku K, Yaginuma Y
Molecular carcinogenesis 2017 May;56(5):1484-1492

---

Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes.

Cheng JC, Tseng CP, Liao MH, Peng CY, Yu JS, Chuang PH, Huang JT, Chen JJW
Scientific reports 2017 Jun 30;7(1):4448

---

Achromatopsia mutations target sequential steps of ATF6 activation.

Chiang WC, Chan P, Wissinger B, Vincent A, Skorczyk-Werner A, Krawczyński MR, Kaufman RJ, Tsang SH, Héon E, Kohl S, Lin JH
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 10;114(2):400-405

---

Dual-Specificity Phosphatase 4 Regulates STAT5 Protein Stability and Helper T Cell Polarization.

Hsiao WY, Lin YC, Liao FH, Chan YC, Huang CY
PloS one 2015;10(12):e0145880

---

Impaired osteoblast and osteoclast function characterize the osteoporosis of Snyder - Robinson syndrome.

Albert JS, Bhattacharyya N, Wolfe LA, Bone WP, Maduro V, Accardi J, Adams DR, Schwartz CE, Norris J, Wood T, Gafni RI, Collins MT, Tosi LL, Markello TC, Gahl WA, Boerkoel CF
Orphanet journal of rare diseases 2015 Mar 7;10:27

---

P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.

Lin YJ, Ho TJ, Lin TH, Hsu WY, Huang SM, Liao CC, Lai CH, Liu X, Tsang H, Lai CC, Tsai FJ
BioMedicine 2015 Jun;5(2):10

---

Long-Term Systemic Exposure to Rotenone Induces Central and Peripheral Pathology of Parkinson's Disease in Mice.

Murakami S, Miyazaki I, Miyoshi K, Asanuma M
Neurochemical research 2015 Jun;40(6):1165-78

---

Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.

Kohl S, Zobor D, Chiang WC, Weisschuh N, Staller J, Gonzalez Menendez I, Chang S, Beck SC, Garcia Garrido M, Sothilingam V, Seeliger MW, Stanzial F, Benedicenti F, Inzana F, Héon E, Vincent A, Beis J, Strom TM, Rudolph G, Roosing S, Hollander AI, Cremers FP, Lopez I, Ren H, Moore AT, Webster AR, Michaelides M, Koenekoop RK, Zrenner E, Kaufman RJ, Tsang SH, Wissinger B, Lin JH
Nature genetics 2015 Jul;47(7):757-65

---

Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture.

Lin YJ, Chen CY, Jeang KT, Liu X, Wang JH, Hung CH, Tsang H, Lin TH, Liao CC, Huang SM, Lin CW, Ho MW, Chien WK, Chen JH, Ho TJ, Tsai FJ
Cell & bioscience 2014;4:40

---

Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer.

Roy D, Mondal S, Wang C, He X, Khurana A, Giri S, Hoffmann R, Jung DB, Kim SH, Chini EN, Periera JC, Folmes CD, Mariani A, Dowdy SC, Bakkum-Gamez JN, Riska SM, Oberg AL, Karoly ED, Bell LN, Chien J, Shridhar V
Cancer & metabolism 2014;2:13

---

Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.

Lee YZ, Yang CW, Chang HY, Hsu HY, Chen IS, Chang HS, Lee CH, Lee JC, Kumar CR, Qiu YQ, Chao YS, Lee SJ
Oncotarget 2014 Aug 15;5(15):6087-101

---

Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.

Law CH, Li JM, Chou HC, Chen YH, Chan HL
Toxicology 2013 Jan 7;303:54-71

---

Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.

Fan CY, Chou HC, Lo YW, Wen YF, Tsai YC, Huang H, Chan HL
Electrophoresis 2013 Dec;34(24):3305-14

---

Proteomic analysis of proteins responsible for the development of doxorubicin resistance in human uterine cancer cells.

Lin ST, Chou HC, Chang SJ, Chen YW, Lyu PC, Wang WC, Chang MD, Chan HL
Journal of proteomics 2012 Oct 22;75(18):5822-47

---

Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.

Cho CK, Smith CR, Diamandis EP
Journal of proteome research 2010 Jul 2;9(7):3574-82

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX101279 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE GTX101279 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE GTX101279 diluted at 1:3000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted by 1:3000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(GTX101279) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin(GTX101279) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. beta Tubulin antibody (GTX101279) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. beta Tubulin antibody (GTX101279) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis. Sample: Paraffin-embedded rat spinal cord. beta Tubulin antibody (GTX101279) diluted at 1:500.