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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- ARG54082 - Provider product page
- Provider
- Arigo
- Product name
- anti-beta Tubulin antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human Tubulin beta protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Goat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E1-E8-H4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Tubulin beta mouse mAb¡¦s stability test under 37¢J 50 hours compare to 4¢J 50 hours with HeLa and Jurkat cell lysate.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and stained with Tubulin beta mouse mAb (diluted in 1: 5000). Predicted band size: 55KDa. Observed band size: 55KDa.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Tubulin beta in goat intestine, goat skeletal muscle, goat spleen and goat lung cell lysates stained with Tubulin beta mouse mAb (diluted in 1: 5000-1: 10000). Predicted band size: 55KDa. Observed band size: 55KDa.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Tubulin beta in Goat skeletal muscle, CHO-k1, COS7, C6, 3T3 and K562 cell lysates stained with Tubulin beta mouse mAb (diluted in 1: 5000). Predicted band size: 55KDa. Observed band size: 55KDa. Exposure time: 10sec.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: beta Tubulin in Chlamydomonas cell lysates stained with beta Tubulin antibody [1E1-E8-H4] (ARG54082) (diluted in 1: 1000). Predicted band size: 55KDa. Observed band size: 55KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 4% paraformaldehyde and stained with Tubulin beta mouse mAb (diluted in 1:500).
