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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB120-11312 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-11312, RRID:AB_2288045
- Product name
- Mouse Monoclonal beta Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. No reactivity with other tubulin isotypes is observed.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Xenopus
- Host
- Mouse
- Antigen sequence
NB 120-11312 specifically recognize
s an epitope located in the C-termi
nal sequence of beta tubulin isotyp
e I of human, bovine, dog, hamster,
rat, mouse, chicken and Xenopus.- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Alpha- and beta-tubulin isotypes are differentially expressed during brain development.
Hausrat TJ, Radwitz J, Lombino FL, Breiden P, Kneussel M
Developmental neurobiology 2020 Apr 15;
Developmental neurobiology 2020 Apr 15;
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-beta-Tubulin I antibody Clone: SAP.4G5. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) Chicken Fibroblasts, 2.) Mouse brain extract I, 3.) Mouse brain extract II, 4.) Rat brain, 5.) Jurkat, 6.) Daudi, 7.) SK-Mel-28, 8.) G361, 9.) HeLa
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Staining of formalin-fixed, paraffin-embedded human testis sections with Monoclonal Anti-beta-Tubulin I, Clone: SAP.4G5 using biotin/ExtrAvidin(R)-Peroxidase.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Staining of formalin-fixed, paraffin-embedded human fallopian tube sections with Monoclonal Anti-beta-Tubulin I, Clone: SAP.4G5 using biotin/ExtrAvidin(R)-Peroxidase.
