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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00203068-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00203068-M04, RRID:AB_1112658
- Product name
- TUBB monoclonal antibody (M04), clone 3B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant TUBB.
- Antigen sequence
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGT
YHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEP
GTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGH
YTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLG
GGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKV
SDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI
CFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQ
LNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ
YRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAA
VFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
TAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQF
TAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATAEEEEDFGEEAEEEA- Isotype
- IgG
- Antibody clone number
- 3B3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Tubulin acetylation alone does not affect kinesin-1 velocity and run length in vitro.
Immune reactivity to β-tubulin isotype 5 and vesicular integral-membrane protein 36 in patients with autoimmune gastrointestinal disorders.
Walter WJ, Beránek V, Fischermeier E, Diez S
PloS one 2012;7(8):e42218
PloS one 2012;7(8):e42218
Immune reactivity to β-tubulin isotype 5 and vesicular integral-membrane protein 36 in patients with autoimmune gastrointestinal disorders.
Op De Beéck K, Van den Bergh K, Vermeire S, Decock S, Derua R, Waelkens E, Rutgeerts P, Nevens F, Bossuyt X
Gut 2011 Nov;60(11):1601-2
Gut 2011 Nov;60(11):1601-2
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TUBB monoclonal antibody (M04), clone 3B3. Western Blot analysis of TUBB expression in Y-79 ( Cat # L042V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TUBB is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TUBB on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
