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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- STJ99132 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-TUBB antibody [1E1-E8-H4] (STJ99132)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Tubulin Beta Chain is suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry research applications.
- Reactivity
- Human, Mouse, Rat, Goat, Hamster, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of beta Tubulin in Chlamydomonas cell lysates using beta Tubulin mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of Tubulin beta in Goat skeletal muscle, CHO-k1, COS7, C6, 3T3 and K562 cell lysates using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size:55KDa.Observed band size:55KDa.Exposure time:10sec.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of Tubulin beta in goat intestine, goat skeletal muscle, goat spleen and goat lung cell lysates using Tubulin beta mouse mAb (1:5000-1:10000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Tubulin beta antibody’s stability test under 37℃ 50 hours compare to 4℃ 50 hours.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
